Create ISO from folder

Folder2Iso just creates an iso from any kind of folder (with the subfolders).
Its a GUI of mkisofs
mkisofs, a CD filesystem image creator. Because these tools don't include any GUI, many graphical front-ends have been created

http://www.trustfm.net/divx/SoftwareFolder2Iso.php

http://en.wikipedia.org/wiki/Cdrtools

Cdrtools - Highly portable CD/DVD/BluRay command line recording software

http://cdrecord.berlios.de/old/private/cdrecord.html